Molecular imaging is a new discipline combining medical imaging technology with molecular biology, chemistry, physics, radiology, nuclear medicine, and computer science. This laboratory mainly studies optical molecular imaging. Compared with imaging modalities such as nuclide, the equipment cost is greatly reduced. Combine specific imaging molecules in the body with imaging targets, and use several imaging modalities such as Optical and micro-CT to perform in vivo, non-destructive and dynamic imaging of small animals, and detect small animal in vivo at the molecular and cellular levels. The physiological and pathological activities of the region of interest , thereby quantitatively obtaining biologically relevant information in the small animal .
 

Imaging algorithm research

In optical molecular imaging, imaging of regions of interest such as lesions is achieved by various reconstruction algorithms. The optical signal distribution of the biological surface obtained by the optical imaging system is combined with the CT system to obtain and use the corresponding algorithm to re-obtain the anatomical structure of the living body, and the specific optical information distribution of each tissue of the living body, and the sense of the living small animal body. The area of ​​interest is imaged. Due to the inherent ill-posedness of optical imaging, its reconstruction algorithm is still an international academic hotspot and difficulty in this field. The laboratory developed a variety of reconstruction algorithms for a series of problems in small animal imaging experiments: multi-level adaptive finite element method, meshless method, Bayesian based method and generalized graph cutting method, etc. The imaging accuracy, depth and speed have been improved to different extents, which promotes the practical application of optical imaging.

The following figures show the process of reconstructing a set of self-illuminating light sources. The light source is below the liver and is about 8 mm from the body surface. Finally, the light source is accurately positioned with an error of less than 0.5 mm.

Full-angle self-luminous signal and mouse two-dimensional white light registration map

  Mouse three-dimensional surface light intensity map

 

 
Software platform construction

The laboratory and Xi'an University of Electronic Science and Technology, American Optical Molecular Imaging Laboratory at Virginia Tech have jointly developed the in vivo optical molecular imaging and biological analysis platform (Molecular Optical Simulation Environment, MOSE) . MOSE is based on the Monte Carlo method to simulate the propagation of photons in biological tissues and free space. The simulation can predict the light intensity signal distribution in the body and surface of small animals. The calculation for this method is very time consuming, and the latest version of MOSE greatly improves the simulation speed. At the same time, it adopts an object-oriented design method, which makes the user-oriented interface simple and easy to understand, and uses Qt to create a software interface. It is currently available free of charge to educational and research institutions and can be downloaded for free on the website .

At the same time, we also provide a set of non-homogeneous digital mouse models imaged by our laboratory micro-CT imaging system, which can also be downloaded for free at the website.

Digital mouse tissue map display in MOSE MOSE simulation mouse surface light intensity distribution

Imaging system development

  The laboratory has independently developed an optical imaging system for obtaining very weak visible light signals emitted from small animals to detect physiological and pathological activities in specific areas of interest in small animals. Meanwhile, micro-CT for small animals has also been developed. The imaging system acquires the anatomical structure information, and can image the tissue and organ as a modality alone, and can be used as a complementary mode of optical imaging to provide geometric prior information for light source reconstruction. This research laid the foundation for the development of multimodal molecular imaging imaging systems.

Optical imaging system micro-CT imaging system

Optical Acquisition and Processing Software CT Acquisition and Reconstruction Software

 

Preliminary in vivo experiment
 
Based on the optical molecular imaging imaging equipment independently developed by our laboratory, we have carried out imaging research and drug efficacy evaluation of liver cancer, breast cancer, nasopharyngeal carcinoma, melanoma and other tumor-bearing mouse models, as well as detection of Luciferase gene in myocardial specificity. The positive expression rate in skeletal muscle-specific transgenic mice achieved the expected experimental results.

The following figure is the imaging effect of cyclophosphamide on the treatment of liver cancer-bearing mice:

Medication group

Control group

Respiratory disease is a common disease, frequently-occurring disease, the main lesion in the trachea, bronchi, lung and chest, lesions are more cough, chest pain, respiratory effects, severe breathing difficulties, hypoxia, and even respiratory failure and death. Due to air pollution, smoking and other factors, The chronic obstructive pulmonary disease is increasing including chronic bronchitis, emphysema, pulmonary heart disease, bronchial asthma, lung cancer, pulmonary diffuse interstitial fibrosis, and pulmonary infection the morbidity and mortality of the disease at home and abroad . Therefore, Anti-Respiratory disease drugs are also more and more attention paid by researchers. Respiratory disease have the symptoms of cough, sputum, hemoptysis, chest pain, shortness of breath and other symptoms of lack of specificity, often clinicians mistaken by people for colds, bronchitis, and severe pneumonia, tuberculosis or lung cancer and other diseases delayed diagnosis; Respiratory tract infection, to be developed to emphysema, pulmonary heart disease, respiratory failure was taken seriously, but it was too late, its pathology and physiological function has been difficult to reverse. As with other systemic diseases, careful and detailed medical history and physical examination are the basis for the diagnosis of respiratory diseases, and should be combined with routine laboratory tests and other special findings to conduct a comprehensive and comprehensive analysis. Currently the most clinical application of anti-respiratory disease drugs have the following four categories: 1. Antihistamines 2. Cough medicine 3. Bronchodilator 4. Expectorant 5. Anti-asthma Drugs.

Anti-Respiratory

Anti-asthma Drugs, Antitussives, Expectorants, β adrenergic receptor agonists, M Cholinergic Blockers, Phosphodiesterase Inhibitors, Anti-Respiratory Drugs,Respiratory System Diseases Treatment

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